Novel high-resolution characterization of ancient DNA reveals C > U-type base modification events as the sole cause of post mortem miscoding lesions

Novel high-resolution characterization of ancient DNA reveals C > U-type base modification events as the sole cause of post mortem miscoding lesions

Ancient DNA (aDNA) research has long depended on the power of PCR to amplify trace amounts of surviving genetic material from preserved specimens. While PCR permits specific loci to be targeted and amplified, in many ways it can be intrinsically unsuited to damaged and degraded aDNA templates. PCR amplification of aDNA can produce highly-skewed distributions with significant contributions from ...

Statistical evidence for miscoding lesions in ancient DNA templates.

It is generally believed that sequence heterogeneity in PCR products from fossil remains are due to regular DNA polymerase errors as well as miscoding lesions compounded by damage in the template DNA (Pääbo 1990; Handt et al. 1994b, 1996; Höss et al. 1996; Krings et al. 1997). However, it has been difficult to test the frequency with which this assumption holds. First, DNA extractions from foss...

Recharacterization of ancient DNA miscoding lesions: insights in the era of sequencing-by-synthesis

Although ancient DNA (aDNA) miscoding lesions have been studied since the earliest days of the field, their nature remains a source of debate. A variety of conflicting hypotheses exist about which miscoding lesions constitute true aDNA damage as opposed to PCR polymerase amplification error. Furthermore, considerable disagreement and speculation exists on which specific damage events underlie o...

the investigation of the relationship between type a and type b personalities and quality of translation

چکیده ندارد.

Recharacterization of ancient DNA miscoding lesions: Insights in the era of the GS20